Review



superscript iii one-step reverse-transcriptase polymerase chain reaction (rt-pcr)  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Qiagen superscript iii one-step reverse-transcriptase polymerase chain reaction (rt-pcr)
    Superscript Iii One Step Reverse Transcriptase Polymerase Chain Reaction (Rt Pcr), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+one-step+reverse-transcriptase+polymerase+chain+reaction+(rt-pcr)/superscript+iii+one+step+reverse+transcriptase+polymerase+chain+reaction++rt+pcr+/pmc06366544-141-13-21
    Average 90 stars, based on 1 article reviews
    superscript iii one-step reverse-transcriptase polymerase chain reaction (rt-pcr) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Clinical Proteomics:

    Article Title: In–Depth Characterization of Viral Isolates from Plasma and Cells Compared with Plasma Circulating Quasispecies in Early HIV-1 Infection
    Article Snippet: .. Total viral RNA was extracted (QIAamp Viral RNA Mini KitTM, QIAGEN, CA) from plasma (2 ml), plasma viral isolates (1 ml), and cell viral isolates (1 ml) in order to carry out PCR amplification. gag , pol, and env -V3 were amplified using one-step reverse transcriptase polymerase chain reaction (RT-PCR) (SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity, Invitrogen, Carlsbad, CA, USA) based on a primer set containing 5′-GCA GAA TGG GAT AGA TTG CAT CCA-3′ (1,417→1,440, HXB2) and 5′-CCT TGT TAT GTC CTG CTT GAT ATT CAC-3′ (5,438←5,464, HXB2), and 5′-TAG AGC CCT GGA AGC ATC CAG GAA G-3′ (5853→5877, HXB2) and 5′-TTG CTA CTT GTG ATT GCT CCA TGT-3′ (8,913←8,936, HXB2) for gag , pol, and env -V3, respectively. ..

    Polymerase Chain Reaction:

    Article Title: In–Depth Characterization of Viral Isolates from Plasma and Cells Compared with Plasma Circulating Quasispecies in Early HIV-1 Infection
    Article Snippet: .. Total viral RNA was extracted (QIAamp Viral RNA Mini KitTM, QIAGEN, CA) from plasma (2 ml), plasma viral isolates (1 ml), and cell viral isolates (1 ml) in order to carry out PCR amplification. gag , pol, and env -V3 were amplified using one-step reverse transcriptase polymerase chain reaction (RT-PCR) (SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity, Invitrogen, Carlsbad, CA, USA) based on a primer set containing 5′-GCA GAA TGG GAT AGA TTG CAT CCA-3′ (1,417→1,440, HXB2) and 5′-CCT TGT TAT GTC CTG CTT GAT ATT CAC-3′ (5,438←5,464, HXB2), and 5′-TAG AGC CCT GGA AGC ATC CAG GAA G-3′ (5853→5877, HXB2) and 5′-TTG CTA CTT GTG ATT GCT CCA TGT-3′ (8,913←8,936, HXB2) for gag , pol, and env -V3, respectively. ..

    Amplification:

    Article Title: In–Depth Characterization of Viral Isolates from Plasma and Cells Compared with Plasma Circulating Quasispecies in Early HIV-1 Infection
    Article Snippet: .. Total viral RNA was extracted (QIAamp Viral RNA Mini KitTM, QIAGEN, CA) from plasma (2 ml), plasma viral isolates (1 ml), and cell viral isolates (1 ml) in order to carry out PCR amplification. gag , pol, and env -V3 were amplified using one-step reverse transcriptase polymerase chain reaction (RT-PCR) (SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity, Invitrogen, Carlsbad, CA, USA) based on a primer set containing 5′-GCA GAA TGG GAT AGA TTG CAT CCA-3′ (1,417→1,440, HXB2) and 5′-CCT TGT TAT GTC CTG CTT GAT ATT CAC-3′ (5,438←5,464, HXB2), and 5′-TAG AGC CCT GGA AGC ATC CAG GAA G-3′ (5853→5877, HXB2) and 5′-TTG CTA CTT GTG ATT GCT CCA TGT-3′ (8,913←8,936, HXB2) for gag , pol, and env -V3, respectively. ..

    Reverse Transcription:

    Article Title: In–Depth Characterization of Viral Isolates from Plasma and Cells Compared with Plasma Circulating Quasispecies in Early HIV-1 Infection
    Article Snippet: .. Total viral RNA was extracted (QIAamp Viral RNA Mini KitTM, QIAGEN, CA) from plasma (2 ml), plasma viral isolates (1 ml), and cell viral isolates (1 ml) in order to carry out PCR amplification. gag , pol, and env -V3 were amplified using one-step reverse transcriptase polymerase chain reaction (RT-PCR) (SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity, Invitrogen, Carlsbad, CA, USA) based on a primer set containing 5′-GCA GAA TGG GAT AGA TTG CAT CCA-3′ (1,417→1,440, HXB2) and 5′-CCT TGT TAT GTC CTG CTT GAT ATT CAC-3′ (5,438←5,464, HXB2), and 5′-TAG AGC CCT GGA AGC ATC CAG GAA G-3′ (5853→5877, HXB2) and 5′-TTG CTA CTT GTG ATT GCT CCA TGT-3′ (8,913←8,936, HXB2) for gag , pol, and env -V3, respectively. ..

    One Step RT-PCR:

    Article Title: In–Depth Characterization of Viral Isolates from Plasma and Cells Compared with Plasma Circulating Quasispecies in Early HIV-1 Infection
    Article Snippet: .. Total viral RNA was extracted (QIAamp Viral RNA Mini KitTM, QIAGEN, CA) from plasma (2 ml), plasma viral isolates (1 ml), and cell viral isolates (1 ml) in order to carry out PCR amplification. gag , pol, and env -V3 were amplified using one-step reverse transcriptase polymerase chain reaction (RT-PCR) (SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity, Invitrogen, Carlsbad, CA, USA) based on a primer set containing 5′-GCA GAA TGG GAT AGA TTG CAT CCA-3′ (1,417→1,440, HXB2) and 5′-CCT TGT TAT GTC CTG CTT GAT ATT CAC-3′ (5,438←5,464, HXB2), and 5′-TAG AGC CCT GGA AGC ATC CAG GAA G-3′ (5853→5877, HXB2) and 5′-TTG CTA CTT GTG ATT GCT CCA TGT-3′ (8,913←8,936, HXB2) for gag , pol, and env -V3, respectively. ..



    Similar Products

    90
    Qiagen superscript iii one-step reverse-transcriptase polymerase chain reaction (rt-pcr)
    Superscript Iii One Step Reverse Transcriptase Polymerase Chain Reaction (Rt Pcr), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+one-step+reverse-transcriptase+polymerase+chain+reaction+(rt-pcr)/superscript+iii+one+step+reverse+transcriptase+polymerase+chain+reaction++rt+pcr+/pmc06366544-141-13-21
    Average 90 stars, based on 1 article reviews
    superscript iii one-step reverse-transcriptase polymerase chain reaction (rt-pcr) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript® iii one-step reverse transcriptase polymerase chain reaction (rt-pcr) system
    Superscript® Iii One Step Reverse Transcriptase Polymerase Chain Reaction (Rt Pcr) System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+one-step+reverse-transcriptase+polymerase+chain+reaction+(rt-pcr)/10__2147_slash_ijn__s82818-46-50-60
    Average 90 stars, based on 1 article reviews
    superscript® iii one-step reverse transcriptase polymerase chain reaction (rt-pcr) system - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript iii one-step quantitative reverse transcriptase-polymerase chain reaction (rt-pcr) kit
    Superscript Iii One Step Quantitative Reverse Transcriptase Polymerase Chain Reaction (Rt Pcr) Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+one-step+reverse-transcriptase+polymerase+chain+reaction+(rt-pcr)/pmc02858320-47-15-25
    Average 90 stars, based on 1 article reviews
    superscript iii one-step quantitative reverse transcriptase-polymerase chain reaction (rt-pcr) kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher superscript iii one-step reverse transcriptase-polymerase chain reaction (rt-pcr) kit
    Superscript Iii One Step Reverse Transcriptase Polymerase Chain Reaction (Rt Pcr) Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+one-step+reverse-transcriptase+polymerase+chain+reaction+(rt-pcr)/mMESSAGE+mMACHINE+SP6+Transcription+Kit/pm18098063-34-10-22
    Average 90 stars, based on 1 article reviews
    superscript iii one-step reverse transcriptase-polymerase chain reaction (rt-pcr) kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results